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By Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)

The annual assembly of the ecu Tissue tradition ., Society was once held on the citadel of Zinkovy in Czechoslovakia from could 7-10,1969. incorporated as a part of this assembly was once a symposium on "Aging in cellphone and Tissue Culture." This quantity includes the papers awarded at that symposium. using mobile and tissue tradition recommendations to check the mechanism of getting older isn't really new. for instance, it has lengthy been identified that age-associated alterations which take place in plasma can inhibit phone proliferation in vitro; additionally that the time lapse sooner than cellphone migration from ex­ planted tissue fragments raises with expanding age. those are either examples of the expression in vitro of getting older in vivo. extra lately, realization has been thinking about the prevalence of senescence in vitro. those investi­ gations have integrated stories of changes in non­ dividing mobilephone cultures, and to a a bit of larger volume, of age-related alterations within the proliferative skill of cells in vitro. for instance, cells derived from human fetal lung preserve many houses of ordinary cells together with a strong general diploid karyotype and those cultures were proven to have a restricted life-span in vitro. In addi­ tion, cultures derived from human grownup lung express a similar general features and seem to have a shorter existence­ span than cells derived from fetal lung.

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Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969

The yearly assembly of the ecu Tissue tradition . , Society used to be held on the fortress of Zinkovy in Czechoslovakia from could 7-10,1969. incorporated as a part of this assembly used to be a symposium on "Aging in mobile and Tissue tradition. " This quantity includes the papers provided at that symposium. using mobilephone and tissue tradition suggestions to check the mechanism of getting older isn't new.

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Additional resources for Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969

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The number of doublings of these cells can be increased by lengthening the growth periods between subcultivations. 5. Parallel cultures of these cells agree very closely in aging phenomena. Furthermore, it appears that the method of cell size analysis can produce useful criteria for the physiological age of aging cells in vitro when the culture conditions can be kept constant throughout the whole lifespan. The data indicate induction of changes into all the cells simultaneously rather than cell selection.

1. The latent period of the spleen, kidney, heart, liver, and brain and the percentage of growing fragments (ordinate). Days of cultivation (abscissa). period is longer) than do the spleen, kidney and liver explants. In adult rats, there is no cell migra- tion in heart and brain under these culture conditions; however, the three more actively growing organs have begun to differentiate, the spleen being the most active organ with the shortest latent period, followed by kidney and then liver. the latent period In old rats, the duration of increases further with no change in 44 M.

I. M. SIMONS On the whole a gradual increase with passage number can be seen. Aging phenomena seem to be present not only during the last passage but even in passages where the cells grow abundantly and look very healthy under the microscope. Therefore, the increase em x s) could be a useful indicator of the physiological age of cell populations. As a possible explanation for increase in mean cell size with aging, an increase in the number of tetraploid or other polyploid cells has been postulated [3].

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